Olympus FluoView FV1000 Confocal Microscope | Confocal Imaging Facility

Overview

The Olympus FluoView FV1000 Confocal Microscope is a laser scanning biological microscope. It minimizes specimen damage during high-speed imaging of living organisms. It accurately captures a full range of related information.

Capabilities

This system enables time-resolved spectroscopy of living intercellular contents.
It features simultaneous application of two laser scanners (SIM or SIMultaneous scanner) to enable synchronization of specimen excitation and observation.
Wavelengths range from UV to IR.
It can analyze objects down to a 2 nm wavelength resolution.
Images up to 4096 x 4096 pixels.

Vendor

Olympus offers a brochure related to the FV1000.

Training

To gain access to the CIF, potential users should either complete the course OR take a specialized training session.

Who should get training?

Training is meant for people who have a solid understanding of confocal microscopy, including the preparation of the samples and dye selection. The purpose of training is solely to give the user experience running the instrument and software.

Who should take the course?

The class is intended to give the student a much broader education on the topics involved with confocal microscopy, including sample preparation techniques and dye selection.

Courses

"Confocal Microscopy" (CH 4800-01/CH 6800-01) is a Special Topics Course designed to provide a solid background on the use of confocal microscopy, as a tool to study cell and molecular biology. The course will consist of lectures that provide theoretical background in various aspects of confocal microscopy, and sessions that give the student hands-on experience in operating a confocal microscope. The course is offered in Fall Semester and includes laboratory training on using confocal microscopy. NO additional training to access confocal facility is required upon passing the course.

CH 4800 - Current Topics in Undergraduate Chemistry

Covers chemistry topics not included in regular courses. Topics may include designing organic syntheses, heterogeneous catalysis, homogeneous catalysis, solid-state chemistry, and heterocyclic chemistry.

Credits: variable to 3.0; Repeatable to a Max of 12
Semesters Offered: On Demand

CH 6800 - Current Topics in Graduate Chemistry

Discussion of recent topics in chemistry at a graduate level.

Credits: variable to 3.0; Repeatable to a Max of 12
Semesters Offered: On Demand
Restrictions: Must be enrolled in one of the following Level(s): Graduate

Other Courses

BL 4035 - Bioimaging

Current concepts in light and electron microscopy and scanning probe techniques. Theory and practice of fluorescence (including confocal and multi-photon), atomic force, scanning and transmission electron, and video microscopy as applied to biological specimens with emphasis on sample preparation. Half semester course.

Credits: 2.0
Lec-Rec-Lab: (0-4-0)
Semesters Offered: Fall, in even years
Restrictions: Must be enrolled in one of the following Class(es): Junior, Senior

BL 5035 - Bioimaging

Current concepts in light and electron microscopy and scanning probe techniques. Theory and practice of fluorescence (including confocal and multi-photon), atomic force, scanning and transmission electron, and video microscopy as applied to biological specimens with an emphasis on sample preparation. Emphasis will be placed on application of advanced techniques. Half semester course.

Credits: 2.0
Lec-Rec-Lab: (0-4-0)
Semesters Offered: Fall, in even years
Restrictions: May not be enrolled in one of the following Class(es): Freshman, Sophomore, Junior

Specialized Training Session

To gain access the same semester to the confocal facility, arrange to get training. No training will be provided in the absence of required forms. Fill out the Olympus FV1000 Training Request Form to negotiate a training session.

Please wait after submitting to be contacted for training session arrangement details.

Before submitting this request form, users must have already filled out the CIF User Profile/Responsible Agreement Form and had it signed by the Account Holder. Present that printed form AT THE FIRST TRAINING SESSION.

Informal Training

Olympus Life Science Solutions maintains a learning center on microscopy topics, including Concepts in Confocal Microscopy.