The Veeco Dimension 3000 provides various modes of atomic force microscopy.
Veeco Dimension 3000 AFM
Overview
Atomic force microscopy is a subset of scanning probe microscopy. The Veeco Dimension
3000 operates in both tapping and contact modes, with capabilities of topographic
and phase imaging, lateral force microscopy, magnetic force microscopy, and adhesion
force mapping. It is also used in measurements of surface and adhesion forces.
Capabilities
The Veeco Dimension 3000 atomic force microscope (AFM) is used to analyze a variety
of sample sizes and types. It can function in air or liquid, making it useful for
biological studies. The sample is placed on a stage which moves in the x, y, and z
directions via piezoelectric elements. A cantilever with a microscopic tip moves like
a spring over the surface of the sample. A laser beam reflected off the end of the
cantilever is recorded on photodiodes, producing an image of the surface topography.
Cantilevers vary in size from 3-100 µm with tip radii of 10-30 nm. The size of the
tip and flexibility of the cantilever contribute to the high resolution of the image,
which can reach 10 p.m. The operating modes of the AFM (contact, non-contact, and
tapping) and lateral force microscopy allow the user to access a variety of functions
of the microscope. In addition to imaging, the AFM can measure the strength of inter-atomic
forces and can sense the presence of individual surface atoms.
SOP
Cantilevers are located in a box in the AFM lab. There is a log sheet next to them.
Facility users must record their use of tips.
General purpose tapping mode cantilevers are available for $21 each. Users will be
charged for a new training cantilever. Afterwards, users can purchase any other type
of cantilever themselves. Contact staff for a vendor list.
Training
Courses
BL 4035 - Bioimaging
Current concepts in light and electron microscopy and scanning probe techniques. Theory and practice of fluorescence (including confocal and multi-photon), atomic force, scanning and transmission electron, and video microscopy as applied to biological specimens with emphasis on sample preparation. Half semester course.
Credits:
2.0
Lec-Rec-Lab: (0-4-0)
Semesters Offered:
Fall, in even years
Restrictions:
Must be enrolled in one of the following Class(es): Junior, Senior
BL 5035 - Bioimaging
Current concepts in light and electron microscopy and scanning probe techniques. Theory and practice of fluorescence (including confocal and multi-photon), atomic force, scanning and transmission electron, and video microscopy as applied to biological specimens with an emphasis on sample preparation. Emphasis will be placed on application of advanced techniques. Half semester course.
Credits:
2.0
Lec-Rec-Lab: (0-4-0)
Semesters Offered:
Fall, in even years
Restrictions:
May not be enrolled in one of the following Class(es): Freshman, Sophomore, Junior