FIB Alignment

Image parameter set

1. Select the following image parameters:

  • Area: 256 μm
  • Zoom: x1
  • Speed: Rapid
  • Accum.: 1

Iext extraction current

2. Open the LMIS tab and check that the extraction current Iext is around 3.2–3.4 μA. If not, use the slider to adjust it to that level.


Trackball with x and y directions marked

3. Using the trackball, locate an area that is suitable for aligning the beams you will be using for your FIB work. Choose an area that can be sacrificed by beam damage during the alignment. This area should be stable and conductive, with some surface topography.


Column adjustment

4. Open the Column Adjustment menu at the top of the screen.


Beam Setting

5. Start by choosing the M0–50 beam in Beam Name under Beam Setting in Column Adjustment:


M0-50 beam setting

Patterning Users Lift Out Users
M0–20 Beam–01
M0–50 M0–20
M1–50 M0–50
M1–100 M1–50
  M1–100
  M1–200
  M1–300
  M1–500

SCAN button

6. Press the Continuous Scan button.


Deflector Shift X and Y

7. Reset the Deflector Shift X and Y to zero.


Aligner X and Y

8. Set the scan Area x Zoom to 256 x 4 to visualize the feature of interest, and then focus.

While focusing, check image for directional twisting (stigmation) and lateral shifting. First correct lateral shifting with the Aligner X and Y with Wobbler: ON.


Stigmator XX and XY

Next, correct stigmation using Stigmator XX and YY. Make small adjustments to improve the image quality, and then recheck the focus to see whether the stigmation is gone.


Contrast and Brightness

9. Set the Contrast and Brightness as necessary.

NOTE: You may need to move the specimen constantly when aligning beams with high currents to prevent erosion of the specimen.


Register button

10. Click Register to save the beam conditions after aligning each beam. Note the beam current for the beam you aligned. It should be between the Max and Min for that setting; it is acceptable if the beam current is over the Max.


11. Go on to the next Beam Name and repeat until you have aligned all required beams for your project.


NOTE: NEVER MAKE CHANGES TO THE APERTURE OR LENS MODE SETTINGS.

Operating Procedure: Lift Out Technique