NOTE: Drift is always down. Use Speed: RAPID.
- Load specimen and focus. Record the focus voltage from the Column Adjustment menu.
- Pull the specimen HOLDER rod into the detent position. (See Step 4 of the Lift Out Technique in Operating Procedures under Hitachi FB-2000A FIB.)
- Insert the depo gun and focus on the depo gun nozzle. The depo gun should be visible in the top right hand corner of the viewing screen at area: 256. Record the focus voltage.
- 3–5 coarse clicks to the left of the focus slider should return you to the recorded specimen focus.
- Move the depo gun out and then in again to check positioning. Finally move the gun out.
- Put both the specimen back and the depo gun back in for a final check.